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All data and code are available upon reasonable request. Calorie-rich diets induce hyperphagia and promote obesity, although the underlying mechanisms remain poorly defined. Nevertheless, how a high-fat diet HFD affects the central regulation of feeding remains largely elusive. The extraction of RNA from precipitated ribosomes allowed for the comparison of mRNA expression both in the input RNA and following pull-down of pS6-bearing ribosomes under both diet conditions. Empty and filled bars represent active and silent cells, respectively. H Quantification of inward rectification to 5 consecutive stimuli as illustrated by the non-linear relationship solid red line compared to a theoretical linear ohmic relationship dashed black line. We next aimed to investigate the response of these cells to nutrients and circulating adiposity signals such as glucose and leptin. To this end, we decreased extracellular glucose concentrations during electrophysiological recordings stepwise from 5 to 0. Altering extracellular glucose concentrations stepwise from 5 to 0.